Ammonium and methyl ammonium transport by RhAG

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چکیده

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Substrate specificity of Rhbg: ammonium and methyl ammonium transport.

Rhbg is a nonerythroid membrane glycoprotein belonging to the Rh antigen family. In the kidney, Rhbg is expressed at the basolateral membrane of intercalated cells of the distal nephron and is involved in NH4+ transport. We investigated the substrate specificity of Rhbg by comparing transport of NH3/NH4+ with that of methyl amine (hydrochloride) (MA/MA+), often used to replace NH3/NH4+, in oocy...

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(Methyl)ammonium transport in the nitrogen-fixing bacterium Azospirillum brasilense.

An ammonium transporter of Azospirillum brasilense was characterized. In contrast to most previously reported putative prokaryotic NH4+ transporter genes, A. brasilense amtB is not part of an operon with glnB or glnZ which, in A. brasilense, encode nitrogen regulatory proteins PII and PZ, respectively. Sequence analysis predicts the presence of 12 transmembrane domains in the deduced AmtB prote...

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pH sensitivity of ammonium transport by Rhbg.

Rhbg is a membrane glycoprotein that is involved in NH(3)/NH(4)(+) transport. Several models have been proposed to describe Rhbg, including an electroneutral NH(4)(+)/H(+) exchanger, a uniporter, an NH(4)(+) channel, or even a gas channel. In this study, we characterized the pH sensitivity of Rhbg expressed in Xenopus oocytes. We used two-electrode voltage clamp and ion-selective microelectrode...

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Ammonium (methylammonium) transport by Azospirillum spp

In recent years soil bacteria of the genus Azospirillum found increasing biological and ecological interest because of their ability to microaerobically fix N 2 and to live in close association with plant roots, stimulating growth of nitrogenlimited plants [1-5]. The physiology of N 2 fixation, especially its repression by N H + , has been studied extensively [6-9]. The question, however, how N...

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Chronic Hyperkalemia Impairs Ammonium Transport

Introduction Previously we demonstrated in rats that chronic hyperkalemia had no effect on ammonium secretion by the proximal tubule in vivo but that high K+ concentrations inhibited ammonium absorption by the medullary thick ascending limb in vitro. These observations suggested that chronic hyperkalemia may reduce urinary ammonium excretion through effects on medullary transport events. To exa...

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ژورنال

عنوان ژورنال: The FASEB Journal

سال: 2012

ISSN: 0892-6638,1530-6860

DOI: 10.1096/fasebj.26.1_supplement.882.8